Protein Purification Reagents and Kits
System Biosciences LLC EXOQUICK-TC FOR TC MEDIA 10MLExoQuick-TC = 10 mL
Fast, scalable exosome isolation from tissue culture media. Need exosomes? SBI's ExoQuick-TC enables high-throughput, quantitative isolation of exosomes from low volumes (as little as 1 ml) of tissue culture media and certain biofluids (saliva, urine, follicular fluid, and breast milk). Note, to isolate exosomes from serum, plasma, or ascites fluid, use the original ExoQuick formulation (EXOQ5A-1 or EXOQ20A-1). Compatible with a wide variety of downstream applications, ExoQuick-TC is an effective and proven alternative to ultracentrifugation.
KAYAKU ADVANCED MATERIALS INC MICROPOSIT 1165 Remover, Clear colorless liquid, 4 x 4 L/CS, Solubility in water: Complete, Hazard classification: Combustible liquid, Flammable liquid, NMP concentration: 94-95%
MICROPOSIT 1165 Remover, Clear colorless liquid, 4 x 4 L/CS, Solubility in water: Complete, Hazard classification: Combustible liquid, Flammable liquid, NMP concentration: 94-95%
STEMCELL Technologies EasySep HLA Chimerism Whole Bl, 60 mL buffy coat or whole blood processed
Column-free immunomagnetic positive selection of human B cells for HLA analysis
AdipoGen Cochlin hLCCL domainrec
Protein. Human cochlin LCCL domain aa 25-132 is fused at the N-terminus to a FLAG -tag. Source HEK 293 cells. Endotoxin content <0.01EU/µg purified protein LAL test Lonza. Lyophilized. Contains PBS. Purity >95% SDS-PAGE. Cochlin is an extracellular matrix protein expressed in the cochlea and vestibule of the inner ear. Mutations in the cochlin gene are linked to the autosomal-dominant DFNA9 syndrome, a disorder characterized by hearing loss. Cochlin is also expressed in follicular dendritic cells of the spleen and the lymph nodes. The N-terminus of cochlin contains a LCCL domain also present in Factor C, a serine protease of the horseshoe crab Limulus, involved in activating the coagulation cascade in response to LPS. A recent study showed that in response to inflammation, LPS or bacterial infection, cochlin is cleaved by the proteases aggrecanases and secreted into blood circulation.
Hampton Research PEG/ION SCREEN NO36 185ML
HR2-922-36/PEG/Ion Screen #36 (C12), 185 ml - 0.2 M Sodium tartrate dibasic dihydrate, 20% w/v Polyethylene glycol 3,350. Please note items from this supplier are non-returnable.
Mayflower Bioscience Exo-spin 20 mini-HD columns
Size exclusion chromatography (SEC) columns are a reliable method for exosome isolation and can be used as a stand-alone product or as part of a combined strategy for isolating small EVs. The EX05 Exo-spin™ kit contains packed and equilibrated ready-to-use SEC columns.
AdipoGen VEGF-C rat rec His
Protein. Rat VEGF-C 127aa is fused at the C-terminus to a His-tag. Source Sf9 cells. Lyophilized. Purity >90% SDS-PAGE. VEGF-C forms primarily non-covalently linked dimers. In adults, VEGF-C is highly expressed in heart, placenta, ovary and small intestine. It binds and activates VEGFR-2 KDR/FLK1 and VEGFR-3 FLT4 receptors. It is a growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. It is involved in the regulation of the growth and/or differentiation of lymphatic endothelium. It is also a mitogen for vascular endothelial cells.
Biotium Indo-1, AM Ester, 10x100 ug
Indo-1, AM ester is membrane-permeant and thus can be loaded into cells via incubation. Because of the relatively low water solubility of the AM ester, Pluronic F-127, a mild detergent, is often used as a dispersing agent to facilitate cell loading. Indo-1/AM itself does not bind Ca2 , but it is readily hydrolyzed to Indo-1 by endogenous esterases once it enters cells. Properties: Light yellow solid soluble in DMSO; Store at -20°C and protect from light, especially in solution; C47H51N3O22; MW: 1010; [112926-02-0]
STEMCELL Technologies EasySep™ Mouse CD4+CD62L+ T Cell Isolation Kit, For processing: 1 x 10^9 cells
Isolate highly purified naïve CD4+ T cells from single-cell suspensions of splenocytes or other tissues by using a simple, two-step method. First, naive CD4+ T cells are pre-enriched by negative selection using the EasySep™ Mouse Naïve CD4+ T Cell Pre-Enrichment Cocktail containing biotinylated antibodies directed against non-naïve CD4+ T cells (CD8, CD11b, CD11c, CD19, CD24, CD25, CD45R, CD49b, TCRγ/δ, TER119). Then CD4+CD62L+ cells, pre-labeled with CD62L PE, are selected using the EasySep™ PE Selection Cocktail. The desired cells are labeled with antibodies and magnetic particles. The cells are separated without columns using an EasySep™ magnet.For the isolation of mouse naïve CD4+ T cells in as little as 15 minutes by negative selection, we recommend using the EasySep™ Mouse Naïve CD4+ T Cell Isolation Kit (19765).
STEMCELL Technologies RoboSep™ Human Myeloid DC Enrichment Kit, For processing: 2 x 10^9 cells
The EasySep™ Human Myeloid DC Enrichment Kit is designed to isolate myeloid dendritic cells (mDC) from fresh peripheral blood mononuclear cells or ammonium chloride-lysed leukapheresis by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-mDCs and dextran-coated magnetic particles. The labeled cells are separated using the EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.
STEMCELL Technologies EasySep™ Human CD34 Positive Selection Kit II, For processing: 5 x 10^9 cells
The EasySep™ Human CD34 Positive Selection Kit II is designed to isolate CD34+ cells from fresh or previously frozen bone marrow or mobilized peripheral blood mononuclear cells, or from previously frozen cord blood mononuclear cells, or from hESC/hiPSC-derived hematopoietic cells by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD34 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD34 antigen is expressed on hematopoietic stem and progenitor cells.This product can be used in place of the EasySep™ Human CD34 Positive Selection Kit (Catalog #18056) for even faster cell isolations.
STEMCELL Technologies EasySep™ Release Human CD3 Positive Selection Kit, 1 x 10^9 cells
EasySep™ Release Human CD3 Positive Selection Kit, 1 x 10^9 cells
STEMCELL Technologies EasySep™ Non-Human Primate CD4+ T Cell Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Non-Human Primate CD4+ T Cell Isolation Kit is designed to isolate CD4+ T cells from fresh peripheral blood mononuclear cells by immunomagnetic negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.This kit has been verified for use with rhesus and cynomolgus macaques.
STEMCELL Technologies RoboSep™ Human Gamma/Delta T Cell Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Human Gamma/Delta T Cell Isolation Kit is designed to isolate gamma/delta T cells from fresh or previously frozen peripheral blood mononuclear cells by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-gamma/delta T cells and dextran-coated magnetic particles. The labeled cells are separated using the EasySep™ magnet without the use of columns. The desired cells are poured off into a new tube. To obtain a custom kit optimized for culture-expanded PBMCs, contact STEMCELL Technologies’ Technical Support at techsupport@stemcell.com.